FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004858409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23572017
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT536210.22747735164114297No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA506710.2149625125418839No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG408310.173218100088762No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC302520.1283386143833173No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA286400.12149999722128148No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG283280.12017639389959713No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGAGCAATCTCGTAT279930.11875521725612195TruSeq Adapter, Index 12 (97% over 37bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA272630.11565832486884765No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG252380.10706763023291559No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)