FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004858413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26794318
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT588690.21970702893053667No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA557570.2080926262053022No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG404330.15090139633335695No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC367300.1370813020880024No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG336860.12572068451229101No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT315950.1179167911644551TruSeq Adapter, Index 22 (97% over 37bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA308520.11514381519245984No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)