FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004858415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64179677
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1412400.22006966473203035No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1332290.20758752026751398No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG977710.1523395014904796No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC871410.13577662598707063No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG811220.1263982677881037No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGGACATCTCGTAT774590.12069085358594124TruSeq Adapter, Index 22 (97% over 37bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA738560.11507692692189772No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)