FastQCFastQC Report
Wed 11 May 2022
EGAF00004858427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30839454
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA771170.25005955034093663No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT758660.2460030582902019No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG566040.18354410554739395No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC537590.17431890979652234No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG401790.13028440775897004No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA387780.12574152577409445No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAA384780.12476874590581274No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG367590.11919471726055851No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC353750.11470695946821886No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA345660.11208369642341917No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTAT334750.10854602030243465TruSeq Adapter, Index 2 (97% over 36bp)
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG334720.10853629250375185No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC311190.10090645573686227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)