FastQCFastQC Report
Tue 5 Jul 2022
EGAF00004858493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160830855
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCCATATCTCGTAT3346860.20809812893178986TruSeq Adapter, Index 16 (97% over 39bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2415690.15020065645985653No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2243950.13952235719943165No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1841000.11446808511961215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)