FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004858505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91034065
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2539470.27895821196164317No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2143580.23547009572735217No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1667320.183153416251378No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1349020.1481884830694971No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG1254890.13784839773990099No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC1131340.12427655515547943No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1035360.11373324919633106No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAACGCCATCTCGTAT963020.10578677333589355TruSeq Adapter, Index 1 (97% over 39bp)
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC956470.10506726245828965No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC916200.10064364367338753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)