FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27256165
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG41000.031.88905743
CGTATGC44200.029.65839644
TATGCCG44550.029.65769446
GATCGGA57200.023.9008771
CTCGTAT54650.023.67154542
ACACGTC62000.022.8181513
CACGTCT76750.018.61273814
ACGTCTG78500.018.0617615
ATGCCGT77650.017.41532947
TCTCGTA72700.017.41474541
ATCGGAA80050.017.0668282
ATCTCGT77950.016.55169940
TATCGCG9700.016.35946822
CACACGT89600.015.98189912
TCGGAAG90850.015.2278433
CGTCTGA98800.014.66477816
GCCGTCT92750.014.20822149
GTATGCC99000.013.76411245
GCACACG112050.013.48856311
CATCTCG98150.013.46156639
Produced by FastQC (version 0.11.5)