FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27256165
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG9050.017.65856622
CGTATCA24800.015.34151950
TTACGCG15100.013.94825754
CGTATCG24400.013.0869993
CGCCGTA29700.012.46729147
GCGGTAT44700.012.2656571
GTATCGA25550.012.232044
TACGCGA17800.012.02337955
GCGTCGT32550.011.4800712
GCCGTAT32450.011.20136848
CCCGTAT28500.010.8734921
ATCGAAG55650.010.7435916
CCGGGAT54300.010.1598591
GTTACGC22850.010.1094453
GATCGGA38800.09.9810252
CCGACAT49300.09.9469071
CGACCTA19200.09.9080644
GCGGAAT31000.09.667041
CGTTCGC16200.09.64596416
ACGCGAC22900.09.64237256
Produced by FastQC (version 0.11.5)