FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43941108
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA42650.019.56419250
GCGTCGT50250.016.90176212
CGCCGTA54050.015.37658947
GCCGTAT55700.014.79644348
TTACGCG30650.014.33385354
GATCGGA64450.013.6151742
TACGCGA35500.012.5690255
AGAGCGT77100.011.6371879
GCGGTAT55700.011.0577451
CCGTATC78500.010.84999249
CCGGGAT87750.010.6276241
AGCGTCG81150.010.55121911
CGTTCGC33100.010.53531216
ACGCGAC42100.010.35393856
TCGTGTA87650.010.08413315
GTTACGC45750.010.05155953
CGTGTAG92700.09.79557816
AGATCGG85900.09.7222451
CGCGGAT39350.09.6389621
GTAGATC88700.09.62475732
Produced by FastQC (version 0.11.5)