FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39687917
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACT736390.18554513707534714No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGG640060.16127326611774562No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAA512950.12924588609676846No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGTGAAAG498610.12563269571441604No Hit
CACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGT471240.11873639022173926No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTA456020.11490146988565814No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA450260.11345014655216096No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATT442780.11156544194546668No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTA433350.10918940391857804No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAAC400090.10080901953105777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGA85000.030.88841411
CGTCGAT90900.028.9610712
GGCTCGT107850.025.6531264
CGTGCGT106400.025.1579028
CTCGGCT135350.025.0289481
GCGTAGC91750.024.47691210
AGTGTCG93250.023.86449251
GTGCGTT94800.023.1809341
AGAACGC118900.022.77142121
GCTCGTG124700.022.6283115
GGTCGCC90950.022.2638725
TCGTGCG126300.022.0116257
ATCATCG123850.021.97834668
CGAGAAT125550.021.91245337
AGTGCGT99900.021.90239340
GTGTCGA102450.021.7554752
CGTTCGA102850.021.5289744
TCATCGA127100.021.5040469
TCGCTAT291350.021.456228
TTGGCCG290350.021.24444841
Produced by FastQC (version 0.11.5)