FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23258231
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23100.013.1743521
TTACGCG8650.013.15830454
TATCGCG5350.012.8936422
CGTATCA19450.011.88104250
GTATCGA19550.010.7617374
GCGGTAT31500.010.7590541
CGTATCG20200.010.7569333
GTCGCTA18200.010.4229527
TACGCGA11400.010.28672455
CCGACAT40800.09.8323291
CCGGGAT50000.09.6139831
CGCCGTA22800.09.53026647
ATCGAAG44400.09.3994946
CTCGGAT49450.09.1614371
TTACACG23250.09.04927332
CGACGAT19500.09.0446991
CGCGAAT8051.8735591E-109.0215671
CGATCCA31700.08.9218284
GCGTCGT25300.08.86123112
CGCCGAT30550.08.6032171
Produced by FastQC (version 0.11.5)