FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21122639
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG206250.055.16327345
CGTATCA202750.054.8553150
GCGTCGT210500.054.3971412
GCCGTAT206550.054.1368248
CGCCGTA208350.053.63759647
TGGTCGC205000.053.58918843
TCGCCGT201500.052.96683546
AGATCGG224100.052.7231331
ACGTACG23000.052.40015826
CCGTATC217050.051.65115449
AGCGTCG224650.051.6031511
TCTCGGT227350.051.28565637
CGGTGGT216750.050.85711340
CGTCGTG226000.050.78972613
GGTCGCC225350.050.40085644
CTCGGTG232650.050.3263938
TCGGTGG234800.050.02314839
GATCGGA245350.049.082942
GTGGTCG225000.048.90226442
GTAGATC231000.048.42865432
Produced by FastQC (version 0.11.5)