FastQCFastQC Report
Fri 3 May 2024
EGAF00004858641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45492225
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT4945261.0870560848584565TruSeq Adapter, Index 16 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA1621160.35635979554748093TruSeq Adapter, Index 16 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG793100.17433748294351398Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)