FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27795111
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA97600.030.0718941
ACACGTC120550.026.5040413
ATCGGAA112850.026.0403612
ACGTCTG130250.024.68724615
GTCACGA52400.024.0602829
CACGTCT134050.023.91139614
AGTCACG54500.023.8913928
ACGATCA58650.021.37901332
TCGGAAG140550.021.338823
CACGATC59450.020.91796731
CACACGT155600.020.4675812
GCACACG161100.020.23797411
CGTCTGA173800.018.61922316
CGATCAG67650.018.58547633
TCACGAT70850.017.5037130
CGGAAGA182650.017.4537184
AATCTCG91700.014.9473741
AGCACAC222550.014.83515710
AGAGCAC227500.014.632688
TCTGAAC228800.014.32359318
Produced by FastQC (version 0.11.5)