FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27795111
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA48850.041.23967412
CATACGA42050.038.88446815
GCATACG51400.037.91798414
ATACGAG49200.027.90478516
CGTATCA37600.027.34512150
ACGGCAT84000.024.14688311
GCCGTAT43400.024.08858948
AGACGGC99650.023.885749
AAGACGG101050.023.6573148
CGCCGTA48450.022.0050947
GCGTCGT49750.021.84159912
GACGGCA106700.020.36767810
TGGTCGC54450.018.94583743
GTCGCCG56850.018.75398345
CCGTATC56800.018.64829449
CGAGATT55950.018.37289619
GAAGACG136300.018.1969437
GGCATAC114350.017.7983813
GATCGGA67500.017.180092
TCGCCGT61100.016.94122346
Produced by FastQC (version 0.11.5)