FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40548322
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG86900.046.13070745
TATGCCG93700.042.93018348
CGTATGC94950.042.4376846
AGTCACG103000.036.80986428
CTCGTAT106700.036.27711544
ACACGTC113600.036.07853313
GATCGGA99750.035.681061
TGAAACG106350.033.73692735
GTCACGT113600.033.28403529
CACGTCT132850.030.9545414
ACGTCTG134600.030.47481715
ACGATCT121550.028.26909439
GAAACGA132850.028.17590336
ATGCCGT142200.028.02116449
ATCTCGT140200.027.2644442
CACACGT155450.026.60965212
CGTGAAA143450.026.04563333
ATCGGAA137750.025.7237682
GCCGTCT154450.025.70935451
AACGATC136200.025.55781638
Produced by FastQC (version 0.11.5)