FastQCFastQC Report
Mon 6 May 2024
EGAF00004858647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17669529
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA2885511.6330429633976093TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT1108260.627215360409437TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)