FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17669529
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG433650.061.5515941
CGTATCA420500.058.938850
GCGTCGT443150.058.39056412
TGGTCGC430600.058.18636743
GCCGTAT427800.058.1099748
GTCGCCG433500.057.99443445
CGCCGTA434850.057.95289247
CCGTATC435450.057.1208849
TCGCCGT424250.057.08893646
TCTCGGT461700.056.87795337
AGCGTCG461300.056.74846611
CGGTGGT446600.056.30272340
GTATCAT439350.056.2683751
GTAGATC453600.056.26277532
GGTCGCC453900.056.1779244
CGTCGTG460200.056.130213
GAGTGTA401500.055.90083328
TCGTGTA449650.055.85890215
TCGGTGG471350.055.84340739
AGAGCGT469400.055.82039
Produced by FastQC (version 0.11.5)