FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38636518
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT73550.011.3730691
TCGCTCG55050.011.18974214
CGCGGGT30600.010.7514951
TCGCGCG10000.010.1498698
CGTTCGC25850.09.61307916
CGAAGAT132350.09.5200891
CGACCGT18500.09.4593371
CGTATCG31900.09.3258983
CTCGGAT89850.09.076121
CGCGAAA17150.08.97966343
CGCTCGT67500.08.97030215
CCGGCAT66400.08.7498871
CGCGAAT18400.08.7498871
CTCGTAT46050.08.7403871
CCCGTAT42900.08.7294911
CGGGGAT79650.08.6125691
CTCGGTT53450.08.4470391
CTCGACT43150.08.4355811
GTATCGA44000.08.3521654
CGCAGAC109200.08.3011751
Produced by FastQC (version 0.11.5)