FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35064986
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60500.020.77158443
TATGCCG63900.019.7220246
CGTATGC68450.018.56347844
CTCGTAT76050.016.4302842
GATCGGA80450.015.3707821
ACACGTC87050.014.83128413
ACGTCTG96050.013.95069715
TATATCG20200.013.51731635
CGTATCG32100.013.4065643
TCGCTCG47150.013.3565614
TTACACG43850.013.17231932
CACGTCT104500.012.78942114
ATCGGAA98350.012.5936582
TCTCGTA99700.012.35756341
CGAAGAT121800.012.3383551
ATCTCGT104200.011.92431840
ATGCCGT112600.011.75174947
CCCGTAT47850.011.3474181
GTATCGA43000.011.3094344
GCCGTCT116250.010.9928249
Produced by FastQC (version 0.11.5)