FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20054899
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG30300.018.43534733
TCGTATG32100.017.30228445
TATGCCG34300.016.14515148
CGTATGC36650.015.11094446
CTCGTAT39950.013.89771144
TCGCTCG25550.012.54966114
GATCGGA48650.012.1409321
GTTTCGG48500.011.80145534
CCGGGAT42500.011.7846821
CGCGATT6201.8189894E-1211.6994761
AATCTCG47050.011.58061541
ACACGTC51850.011.50369313
TAAGTCG15050.011.45485820
AGTCACG51850.011.3716128
TTCGGAA56800.010.98715736
CGGAATC53300.010.93213138
TCTCGTA51150.010.85461443
ACGTTTC53050.010.7894832
GTCACGT55050.010.64845929
TTTCGGA55050.010.58508235
Produced by FastQC (version 0.11.5)