FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20054899
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG29400.013.84232714
TATCGCG5301.09139364E-1011.71620820
CGCAGAC59250.010.9936861
CGCGAAT9550.010.8264911
CGCGGAT19600.010.7261451
CCGGGAT44000.010.4959561
CGACTAA14600.010.16318228
CCCGATA39150.010.14346269
ACTCGTG40200.010.126322
CGAAGAT64500.010.098851
CTCGGAT50900.010.0887741
TACTCGT40500.09.88083121
CGCATAT10500.09.8469521
CTCGTAC42850.09.74148217
ACCCGAT40200.09.70664968
CGCTCGT42900.09.64793115
CGACAAT21150.09.4512121
ACTTCGC45500.09.39165711
CCGAGAT61550.09.18302251
CGCCAAT38850.09.1372611
Produced by FastQC (version 0.11.5)