FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27227094
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG40750.013.54489114
TATCGCG6050.012.54545420
CGTATCA26900.012.31314949
TTACACG34200.012.30941632
GCCGTAT30850.011.29576547
CCGGGAT56000.011.1001391
GATCGGA47850.010.7534621
CGCGATT9150.010.5677111
CGAAGAT89300.010.5186841
ACTCGTG54550.010.30890922
GCGTCGT39250.010.28409511
CGCAGAC84250.010.0834511
CCCGATA55750.09.83808769
CCGAGAT78300.09.7911831
CGCGAAT13850.09.4749691
CGGCAAT41950.09.4669451
CGCCGTA36900.09.43989746
CGCTCGT60300.09.38225615
CGCCAAT50600.09.3500521
CGCGGAT26850.09.2604511
Produced by FastQC (version 0.11.5)