FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27915228
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG36300.015.0413745
CGCGAAA12200.014.34433543
TATGCCG40550.014.15540948
CGTATGC41550.013.39354546
TATCTCG46950.011.77839941
CGCGTAA3903.0234514E-711.6683761
CGCGGGT19550.010.9223171
ACACGTC55600.010.19774613
GATCGGA48850.010.0321761
CTCGTAT58800.09.76193644
TCGGGTC45550.09.6049652
CCGGCAT45850.09.5433831
CTCGGGT61550.09.3271181
ACGTCTG60150.09.30997115
CGCGTAT5301.5323876E-69.2466381
CGCGGAT22050.09.2076981
CCCGTAT31700.08.9445291
ATCTCGT64050.08.90714842
CGTATCG20900.08.8755313
CCGCGAA20550.08.85649242
Produced by FastQC (version 0.11.5)