FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54547832
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG89850.030.67788743
CGTATGC101800.027.21225444
TATGCCG101900.027.15169346
CTCGTAT112550.023.87749342
ACACGTC131550.023.37341313
GATCGGA126450.022.210041
CACGTCT165550.018.6553814
ATGCCGT154700.018.28608347
TCTCGTA153050.017.3336441
ACGTCTG184500.016.84643615
CACACGT188800.016.37757912
ATCTCGT168900.015.56370340
GACCAAT194550.015.1960835
CGTCTGA209600.015.09247516
GCCGTCT188000.015.0835949
TCGGAAG190050.014.9023963
ACCAATC192550.014.33271536
GCACACG230050.014.22119711
TTACGCG40750.014.13822254
ATCGGAA197350.014.0539922
Produced by FastQC (version 0.11.5)