FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54547832
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA44400.030.92094615
GCATACG55400.028.08559814
CGGCATA55550.027.65823212
ATACGAG65750.019.7462116
TTACGCG42100.013.84955554
AGACGGC142250.013.6158819
GCGGTAT92850.013.1936961
ACGGCAT122300.012.93016811
TACGCGA48550.012.56821355
GTATCGA47300.012.0400334
GTCGCTA53050.012.01299227
ATCGAAG114400.011.911986
CGTATCG53950.011.6869463
ACGAGAT107100.011.52105518
TACGAGA111250.011.36551617
CCGACAT103300.010.9391881
CGCGTAT10050.010.8050081
CCCGTAT56550.010.3813711
GACGGCA171350.010.32579310
GGCATAC165250.010.01453513
Produced by FastQC (version 0.11.5)