FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28763193
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA76100.028.9148851
ACACGTC89750.027.02443513
GTCACGA52100.025.05570229
ATCGGAA87900.024.9839132
ACGTCTG101500.024.19947815
AGTCACG57400.023.46228828
CACGTCT106850.022.89214914
ACGATCA59750.021.6751432
TCGTATG74400.020.3156843
CACGATC66050.019.81575231
TCGGAAG115700.019.3006063
CACACGT128450.019.23008212
TATGCCG78600.019.06039646
CGTCTGA128800.018.98982816
TCACGAT71450.018.60660630
CGTATGC81750.018.40774744
GCACACG136050.018.30736511
CGATCAG72050.018.1654633
CTCGTAT89900.016.50728642
CGGAAGA161550.015.6291664
Produced by FastQC (version 0.11.5)