FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31881106
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT19500.011.8487081
TCGCTCG40400.011.26259614
CCGGGAT53600.010.7112641
TATGCCG40300.010.24774446
CGACCGT12250.010.0021551
CGCGTAA4251.0678445E-59.88149210
CCGGCAT48650.09.7143241
TCGTATG41050.09.54854243
CGAAGAT105100.09.2598451
CGTATGC43500.09.252110544
CGCTCGT51050.09.1872515
CGCGAAA12700.09.09373843
CTCGGGT67950.08.9644041
TTACACG34950.08.91395532
TATCTCG49700.08.87302239
CTCGTAT50100.08.87148242
CTCGGAT67600.08.7518861
CGCAGAC78950.08.6909171
CGGGGAT58050.08.6840421
CCGCAAT34800.08.6512891
Produced by FastQC (version 0.11.5)