FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13960716
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA77650.056.09650815
GCATACG85500.054.9951714
CGGCATA82650.054.46993312
ATACGAG73300.052.4134516
ACGGCAT106200.046.8621311
AGACGGC120350.045.08049
AAGACGG121450.044.9243058
TACGAGA65050.042.7316117
GACGGCA116450.041.85373710
GGCATAC111450.040.48711413
GAAGACG143100.039.3808257
TGCCTAA88950.039.1069724
CCTAAGT68300.039.0174126
GCCTAAG98200.038.8286825
ACGAGAT65600.038.64070518
CTAAGTG66150.037.20631427
CGTATCA84100.034.9119150
ATACGCG4250.034.8932216
CAAGCAG233350.034.087641
GCGTCGT89250.032.8935312
Produced by FastQC (version 0.11.5)