FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30341224
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG41100.016.06926546
TCGTATG39900.016.03796443
CGTATGC45100.014.49230244
CTCGTAT50950.012.42537442
ACACGTC56050.011.72198513
GATCGGA53250.011.1476991
TTACACG31850.011.06623132
CGAAGAT94350.010.8739681
AATCTCG60100.010.761239
CCGGGAT59150.010.6158581
ACGTCTG67050.010.61557915
TATCGCG7900.010.39606520
CTCGGAT75950.09.8940721
TCTCGTA66250.09.76242941
CGCGAAT13000.09.5020621
CACGTCT74350.09.48124514
ATCTCGT71950.09.22665540
TCGCTCG42550.09.16820614
ATCGGAA65450.09.1628362
ATGCCGT79900.09.0796647
Produced by FastQC (version 0.11.5)