FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31020053
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA23150.019.2234215
TATCGCG5950.016.81312622
CGGCATA26950.016.76916712
TTACACG39800.016.29575332
GCATACG28000.016.14008914
TCGCTCG53100.015.72220614
TAAGCGA23450.015.2999846
CGAAGAT115400.014.0013711
GTTAACG15000.013.79913815
ATACGAG35400.012.96105516
ACTCGTG65500.012.745048522
CGTATCG21250.012.6722673
GATCGGA53950.011.9665262
CGCAGAC100400.011.8635851
GTATCGA29000.011.7846294
CTCGGAT84650.011.5830231
CGTTCGC25250.011.4765116
ATCGGAA65300.011.261213
CGTATCA31250.011.14970450
CGCTCGT73400.011.1389515
Produced by FastQC (version 0.11.5)