FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36602796
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG51250.034.1207843
CGTATGC52800.032.6618744
TATGCCG56200.030.9315446
CTCGTAT65000.026.42532242
ACACGTC73000.024.05632813
GATCGGA72350.023.6947021
CACGTCT94150.019.09254314
CGTATCG42000.018.2310453
TCTCGTA93050.018.16285141
CCCGTAT39250.017.9276711
ATGCCGT101450.017.7810347
GTATCGA39750.016.9201954
CACACGT105900.016.8439312
TCGGAAG104000.016.8144443
ACGTCTG115650.015.65898715
CGCGTAA36150.015.6483631
GCCGTCT116100.015.388844549
GTCGCTA29250.015.21113627
CCGTATC57350.015.1556032
GCGGTAT63100.015.0326911
Produced by FastQC (version 0.11.5)