FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36602796
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT41100.018.405221
CGTATCG46750.018.1523823
GTATCGA38200.018.1515144
CGCGTAA36100.014.4480251
ATCGAAG84350.014.2323326
GTCGCTA30050.013.66100427
CGGTATT46400.013.0850322
CCGGTAT51900.013.04451
CCGTATC73100.012.8360592
CGTATCA31950.012.41672750
TCGATTG42250.012.24742320
AATCGGG65000.012.2066485
GCGGTAT61350.012.0486381
TTACGCG25200.011.63592354
CGAGCGA77350.011.4172253
CCGACAT64350.011.3795751
TCGAAGG102200.011.3414957
GCGTCGT35950.011.32304612
TTACACG39900.010.98027832
GCGTAAC50300.010.5616772
Produced by FastQC (version 0.11.5)