FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28268960
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG11900.017.71520422
CGTTTCG31550.016.58162143
TTACGCG37250.016.32410454
ATCGAAG69900.015.64872556
TACGCGA39600.015.52699955
GTCGCTA53300.015.23249227
CCGACAT63300.015.2260041
TCGCTAT54150.015.18158728
GAACGCT56800.014.81107635
ACGGACA85350.014.01006618
CGACATC65750.014.0014752
AAGCGAC61150.013.73415520
GCGGTAT71650.013.3565221
ATTCCGT89350.013.34484853
CGCGAGT35600.013.26371211
GTTACGC46900.013.25498753
CGCTATG63350.013.24495829
GTTACGA20800.013.0655274
TTACACG28150.013.03047232
CGGTCTA29200.012.91344144
Produced by FastQC (version 0.11.5)