FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24077025
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA119650.028.9520761
ACACGTC135850.028.949913
ACGTCTG148550.026.56540515
ATCGGAA131550.026.2516062
CACGTCT153250.025.7507814
GTCACGA94950.024.05050829
AGTCACG100350.023.37506328
TCGGAAG150850.023.0758933
CACACGT175600.022.45554412
CGTCTGA176450.022.44298216
GCACACG182350.022.30534611
ACGATCA103800.021.96732
CACGATC105050.021.80412731
TCGTATG126900.021.36634643
CGATCAG111050.020.6571233
CGGAAGA192000.020.5355874
TCACGAT113100.020.34369730
CTCGTAT136100.019.74465642
TATGCCG141050.019.30067446
CGTATGC142200.019.11825644
Produced by FastQC (version 0.11.5)