FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31726277
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA28400.021.0178912
CATACGA23200.019.77984815
CGTATCA38350.019.43019750
GCATACG32800.019.35554114
GTCGCCG38250.019.20448945
GCGTCGT38950.018.69116212
GCCGTAT40100.018.49350448
CGCCGTA45950.015.98761147
TGGTCGC51200.014.48181743
TCGCCGT53050.014.10685846
AGCGTCG52600.014.03600511
TCGCTCG42250.013.80134214
GATCGGA56300.013.3557962
CCGTATC60150.012.3878549
CGCTCGT48050.012.06355215
GGTCGCC61700.011.84971844
CGTATCG23650.011.8132883
AGAGCGT64400.011.783229
CGTCGTG65200.011.48347913
CGCAGAC71200.011.4815081
Produced by FastQC (version 0.11.5)