FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29748717
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50650.017.4148846
TCGTATG52400.017.03341543
CGCGAAC7850.016.5125261
CGTATGC54650.016.26827444
GTCACGA60550.015.08898629
TCGCTCG37550.014.44354114
ACACGTC66050.014.30351713
CTCGTAT66400.014.07455242
AGTCACG68800.013.78842428
ACGATCA72850.012.73626432
CGCTCGT44000.012.72386915
CACGATC74250.012.49588831
ACGTCTG76900.012.37640415
GATCGGA57400.011.9626021
CCGGGAT47400.011.8995081
CGATCAG80900.011.85840633
ATGCCGT78400.011.6968647
CACGTCT84450.011.68426514
TCACGAT82100.011.42754930
CTCGGTT36550.011.4062021
Produced by FastQC (version 0.11.5)