FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37318837
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG59100.016.16978643
TATGCCG62850.015.42809746
CGTATGC66400.014.70827644
ACACGTC88800.011.70187713
GATCGGA81700.011.5770871
AATCTCG82700.011.55555839
CTCGTAT82250.011.44700742
TCGCGCG5207.858034E-1011.4375856
TCGCTCG41250.011.28041714
ACGTCTG96550.010.61705615
CGAAGAT129500.010.3876921
CGCTCGT48050.010.26631215
GTCGTTA24900.010.257977520
CGCGTTC21900.010.22429514
ATGCCGT101800.010.040894547
CGATAAC33900.09.92041
CACGTCT108050.09.87580614
CGCGGGT23350.09.7517841
CGCGAAT14800.09.7046471
CGATCCA55550.09.6989654
Produced by FastQC (version 0.11.5)