FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27039908
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG32700.016.4826333
TCGTATG38000.012.98734545
TATGCCG40550.012.34330848
CGTATGC41600.012.11589646
CGCGTAT4955.2930773E-810.6092561
AGTCACG58850.010.40733228
GATCGGA48450.010.4056111
ACACGTC58350.09.95669313
AATCTCG49350.09.92903941
CTCGTAT50600.09.89161344
CTCGGAT63200.09.7497721
GTTTCGG58350.09.65691734
CGTTCGC18550.09.62308416
CGCGAAA10950.09.5893443
TCGCTCG34750.09.56836114
ACGTTTC62350.09.48634532
GTATCGA27450.09.4348814
TCGGGTC46700.09.2934112
CTCGGGT60700.09.228481
CGCGTTC20500.09.21952714
Produced by FastQC (version 0.11.5)