FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27039908
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA26350.013.81413950
CGTTCGC18800.011.91484616
GCCGTAT30900.011.78001848
GCGTCGT35050.011.18397812
CGCCGTA33550.011.058247
GTATCGA25300.010.3756444
CGCGTTC21600.010.37032914
TCGCTCG37300.010.32167414
TCGGGTC44950.09.9668282
TAAGTCG27300.09.74355120
CGAAGAT92700.09.6657821
TTACACG28450.09.59579732
ACACGCG28700.09.512156569
CTCGGGT61750.09.4657761
CCCGTAT30050.09.4344641
CCGCGTT24900.09.27707113
CGTATCG21150.09.2673253
CGACCTA26800.09.1419234
TCGCGCG8051.4733814E-109.1304498
GTTCGCA26150.09.10130117
Produced by FastQC (version 0.11.5)