FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27309858
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG6900.019.998122
TCGCTCG38700.013.46035614
TAAGCGA19650.012.81637646
CGAAGAT87000.012.4996311
CTCGGGT61750.012.1318951
CGTTCGC17650.011.922708516
CGGGGTT63950.011.6065661
TCGGGTC49950.011.472832
TTACACG30700.011.23706832
CGCAGAC78500.011.0824751
CGCTCGT49850.010.72647815
ACTCGTG50300.010.56162222
CTCGGAT67300.010.2593741
CGGAGAT66000.010.0429931
CGGAAAT48350.09.9962581
TCGTGCG53200.09.98587524
CGTATCA25850.09.87570550
CGCGAAA9800.09.85651443
CTGGAAT221450.09.821341
TTCGCAC22750.09.70473318
Produced by FastQC (version 0.11.5)