FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234182
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34250.014.8057845
TATGCCG39750.012.79858548
CGTATGC40800.012.63938246
TCGCTCG33300.011.70025814
CCGGGAT48250.011.2390481
TAAGCGA13800.010.7842346
TATCGCG6201.5279511E-1010.56658820
CGCGTAT4251.0899148E-610.5652941
CGCGATT7550.010.5222371
CGAAGAT69850.010.3843681
AGTCACG55300.010.35077528
CGCGAAT10050.010.3105641
CGCAGAC79550.010.2470531
CTCGTAT51500.010.24520644
TAAGTCG16550.010.20871320
GATCGGA51750.010.0784521
CGGAAAT48800.010.0506861
ACGATCG4251.248907E-59.7519553
GTCACGT57350.09.68041529
CGCGGAT23050.09.590391
Produced by FastQC (version 0.11.5)