FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29430793
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA318760.10830832862709476TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG148400.036.31170745
TATGCCG149350.035.6880548
CGTATGC152750.035.05177346
CTCGTAT159350.033.12370344
AATCTCG164450.032.64188441
ACACGTC172500.032.47881313
GATCGGA166100.031.6125221
ACGTCTG179450.031.35544415
TCTCGTA170550.030.90801643
ATCTCGT173300.030.3180242
CACGTCT189950.029.71304714
ATCGGAA177250.029.6536732
ATGCCGT182300.029.18080149
GCCGTCT186050.028.57409351
CCGTCTT194500.027.56328252
TGCCGTC198250.026.90269750
CACACGT209700.026.88170612
GCACACG215000.026.50786811
TCGGAAG203150.026.023663
CGTCTGA226050.024.9983816
Produced by FastQC (version 0.11.5)