FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29430793
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA35650.044.90600215
GCATACG48000.042.48075514
CGGCATA48250.041.6169412
CGTATCA81100.032.7995950
GCCGTAT83650.031.95541648
GTCGCCG81000.031.89049345
ATACGAG46600.029.98576216
CGCCGTA89050.029.90006847
GCGTCGT87100.029.23362512
TGGTCGC88000.028.96189143
TCGCCGT93450.027.4579246
CCGTATC103050.026.07701949
AGACGGC87100.025.5432439
AGATCGG106550.024.6055891
GGTCGCC107400.024.27622844
AGCGTCG109850.023.42943811
TCTCGGT113350.023.3919437
ATACGCG6350.023.36365116
GATCGGA116200.023.3330552
CGTCGTG111400.023.19757513
Produced by FastQC (version 0.11.5)