FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42881021
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44100.021.90374843
CGTATGC49300.019.8033244
TATGCCG51300.019.16573746
TTACGCG22750.016.2260454
CTCGTAT60750.016.01407442
ACACGTC72050.013.98103213
TACGCGA26850.013.61983155
GATCGGA61000.012.5685661
CGCGAAT15250.012.4553361
TATCTCG59600.012.44480639
AGTCACG59550.012.10775328
TCTCGTA86400.011.419517541
ACGCGAC33800.011.22750156
CACGTCT93800.011.10694214
ACGTCTG96700.010.6668115
CGTATCG32700.010.5510033
ATGCCGT97800.010.51186947
GTATCGA40150.09.9674754
CCGGGAT85900.09.9303921
GTTACGC38250.09.92134153
Produced by FastQC (version 0.11.5)