FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42881021
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG23250.012.38456954
GCGGTAT49650.011.0488431
TACGCGA26850.010.59637555
CGACAAT44500.010.1520821
CGTATCG32450.010.0514813
CCCGTAT41350.09.1912581
GTATCGA37050.09.096724
CCGGCAT83450.09.0227221
CCGGAAT79200.08.8731021
CGACCTA23350.08.752524
CCGGGAT87450.08.7330191
CGCCGAT63100.08.693741
TTACACG42850.08.67019632
CGAAGAT121850.08.5038771
CCGCAAT48000.08.4407651
CGATCCA65300.08.4008544
CCGACAT65750.08.3978791
TCGACCG34500.08.35357531
ACGCGAC35400.08.32610956
CGCAGAC116700.08.2339561
Produced by FastQC (version 0.11.5)