FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36871779
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG44250.014.68358745
TATGCCG50900.013.09936948
TCGCTCG51500.012.74891914
CGTATGC52800.012.62799146
TTACACG40700.011.4509132
CTCGTAT56350.011.34948144
CGAAGAT118750.010.8436061
CGCAGAC97950.010.6297071
CGCTCGT64850.010.28180515
AACGATC59550.09.87316138
CCGGGAT66050.09.8591571
CGCCAAT62450.09.6027251
AGTCACG68400.09.54898528
CGTATCG24300.09.5220753
CCGGAAT55500.09.4794331
CGGAAAT65300.09.46532251
ACACGTC69850.09.44840713
CAATCGG40350.09.2820914
CTCGGAT81050.09.1693321
GATCGGA65500.09.1555751
Produced by FastQC (version 0.11.5)