FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28887999
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG38350.032.92458345
TATGCCG40750.031.49342748
CGTATGC43400.029.33196846
ACACGTC56500.024.11913
CTCGTAT51800.023.70967544
GATCGGA50550.021.5787811
CGTCCCG49700.018.950435
ACGTCTG71250.018.93215
CACGTCT73750.018.47750914
TCTCGTA70900.017.8090943
ATGCCGT75000.017.24942449
CACACGT79250.017.15171612
ACCCGTC55150.017.14010232
GTCCCGA58800.016.54565236
TCCCGAT61400.015.17076637
ATCTCGT83500.014.83251642
CCGATCT61100.014.73700239
GCCGTCT91900.014.07733251
TCGGAAG80650.014.0746223
GCACACG101750.013.90144811
Produced by FastQC (version 0.11.5)