FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28887999
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG16400.013.81640854
GCGGTAT33000.012.1234371
TACGCGA19750.011.82050855
CCGGGAT63850.010.5701881
CCGACAT53000.010.3054191
ATCGAAG64050.010.2068656
CCCGTAT28250.09.6054561
GTCGCTA32800.09.48459627
TTACACG27550.09.33563132
CCGGAAT60650.09.2923611
GTATCGA26650.09.1369814
CGTATCG30100.09.1302783
CGAAGAT80850.09.122161
GTTACGC25600.09.04907353
ACGCGAC26300.09.00715556
CGGAGAT69600.08.9471691
CGCGGAT21800.08.777041
GCGGAAT35000.08.7469461
ACTCGTG56250.08.60315422
TAGACCG20400.08.58314658
Produced by FastQC (version 0.11.5)