FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004858707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004858707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29682722
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG217200.047.75249543
TATGCCG230500.044.9821546
GATCGGA222050.044.9132961
CTCGTAT223750.044.74923742
CGTATGC233200.044.4175544
ACACGTC243900.044.30574813
AATCTCG231650.042.9710139
ATCGGAA233800.042.524882
TCTCGTA235550.042.42033841
ACGTCTG258100.041.8154815
CACGTCT261200.041.319214
ATCTCGT247400.040.30454340
CACACGT276900.039.18618812
TCGGAAG258250.038.6171843
ATGCCGT267650.038.5468247
ACCAATC266200.037.45789736
GACCAAT275500.037.44753335
GCACACG296350.037.04167611
CGTCTGA291700.037.0338916
GCCGTCT288350.036.0641449
Produced by FastQC (version 0.11.5)